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Hair Growth Efficacy Test
In Vivo Hair growth efficacy test
Hair growth animal model
▪ Species: C3H/HeN
▪ Methods: To make the telogen equal and induce anagen
▪ Measurement
– After promoting anagen, measure hair weight
– Measuring the number of hair follicles after tissue staining
Hair loss animal model
▪ Species: C3H/HeN
▪ Methods: After inducing hair loss by DHT, evaluation the hair growth
▪ Measurement
– measuring hair weight
– Measuring the number of hair follicles after tissue staining
Ex vivo Hair growth efficacy test
▪ Species: C3H/HeN, pig
▪ Methods: To extract mouse vibrissae or pig skin hair follicle, then add to the appropriate medium and incubate in vitro
▪ Measurement
– Hair shaft length
– Anagen elongation
In vitro Hair growth efficacy test
▪ Cell line type: human DPCs, human ORS cells, human ASC, pigDPCs, pigORS cells,
▪ Measurement
– Evaluation paracrine efficacy
– Evaluation of growth factors related to hair growth
