Sox2 Reporter Stable Cell line (For Research Use Only)
Human dermal papilla cell line (catalog number #STC001)
Sox2 is a critical component of the core transcriptional regulatory circuitry that controls pluripotency in ESCs. It is essential for the development of the epiblast in the early mammalian embryo and for the maintenance of embryonic stem cells (ESCs). Sox2 is also necessary for the function and maintenance of neural progenitor cells (NPCs) in the nervous system. Further, Recently, Sox2 was shown to play a role in maintaining adult stem cells in several organ systems. In skin, Sox2 is not expressed in hair follicle stem cells, but is one of the highest expressed transcription factors in the dermal papilla (DP), first identified in a screen of DP signature genes in growing hair follicles. It interacts with DNA by binding to consensus sequence CATTGTG.
STEMORE has developed Sox2 luciferase reporter stable cell line by infecting Sox2 pGreenFire Response Reporter vector. The puromycin resistant clones were subsequently screened for Sox2 Transcription Factor Activity.
One vial of 1 ´ 106 cells. Store the frozen cells in liquid nitrogen until you are ready thaw and propagate them.
Handling and Storage
The cells should be cultured in complete DMEM medium [In 450 mL of high-glucosed DMEM, add 50 mL FBS (10% final) and 5 mL Penicillin/Streptomycin (1% final)].
Initial Culture Procedure
- Quickly thaw cells in a 37°C water bath. Remove from the as soon as the vial is thawed.
- Transfer cells to 100 mm2 dish containing 10 ml of complete growth media.
- Gently rock the dish to ensure the cells are mixed well in the media.
- Place the dish with cells in a humidified incubator at 37°C with 5% CO2.
- After cells adhere (wait at least 4 hours to overnight), replace media with fresh complete growth medium.
- Subculture cells when density reaches 90~100% confluency.
- Carefully remove the culture media from cells by aspiration.
- Rinse cells with PBS, being careful to not dislodge attached cells. Then remove PBS by aspiration.
- Add 1~2 mL trypsin-EDTA solution.
- Incubation with trypsin for 2~5 minutes (or until detached). Confirm detachment by observation under the microscope.
- Add 5~10 mL of pre-warmed complete growth media and gently pipet up and down to break the clump.
- Passage cells in 1:3 to 1:5 ratio when they reach 90% confluency.